Tomato line FDR 15-2090

ABSTRACT

The invention provides seed and plants of tomato hybrid PS01533588 and the parent lines thereof, such as inbred tomato line FDR 15-2090. The invention thus relates to the plants, seeds and tissue cultures of tomato hybrid PS01533588 and the parent lines thereof, and to methods for producing a tomato plant produced by crossing such plants with themselves or with another tomato plant, such as a plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of such plants, including the fruit and gametes of such plants.

FIELD OF THE INVENTION

The present invention relates to the field of plant breeding and, morespecifically, to the development of tomato hybrid PS01533588 and theinbred tomato lines FDR 15-2090 and FDR 15-2078.

BACKGROUND OF THE INVENTION

The goal of vegetable breeding is to combine various desirable traits ina single variety/hybrid. Such desirable traits may include any traitdeemed beneficial by a grower and/or consumer, including greater yield,resistance to insects or disease, tolerance to environmental stress, andnutritional value.

Breeding techniques take advantage of a plant's method of pollination.There are two general methods of pollination: a plant self-pollinates ifpollen from one flower is transferred to the same or another flower ofthe same plant or plant variety. A plant cross-pollinates if pollencomes to it from a flower of a different plant variety.

Plants that have been self-pollinated and selected for type over manygenerations become homozygous at almost all gene loci and produce auniform population of true breeding progeny, a homozygous plant. A crossbetween two such homozygous plants of different genotypes produces auniform population of hybrid plants that are heterozygous for many geneloci. Conversely, a cross of two plants each heterozygous at a number ofloci produces a population of hybrid plants that differ genetically andare not uniform. The resulting non-uniformity makes performanceunpredictable.

The development of uniform varieties requires the development ofhomozygous inbred plants, the crossing of these inbred plants, and theevaluation of the crosses. Pedigree breeding and recurrent selection areexamples of breeding methods that have been used to develop inbredplants from breeding populations. Those breeding methods combine thegenetic backgrounds from two or more plants or various other broad-basedsources into breeding pools from which new lines and hybrids derivedtherefrom are developed by selfing and selection of desired phenotypes.The new lines and hybrids are evaluated to determine which of those havecommercial potential.

SUMMARY OF THE INVENTION

In one aspect, the present invention provides a tomato plant of thehybrid designated PS01533588, the tomato line FDR 15-2090 and tomatoline FDR 15-2078. Also provided are tomato plants having all thephysiological and morphological characteristics of such a plant. Partsof these tomato plants are also provided, for example, including pollen,an ovule, scion, a rootstock, a fruit, and a cell of the plant.

In another aspect of the invention, a plant of tomato hybrid PS01533588and/or tomato lines FDR 15-2090 and FDR 15-2078 comprising an addedheritable trait is provided. The heritable trait may comprise a geneticlocus that is, for example, a dominant or recessive allele. In oneembodiment of the invention, a plant of tomato hybrid PS01533588 and/ortomato lines FDR 15-2090 and FDR 15-2078 is defined as comprising asingle locus conversion. In specific embodiments of the invention, anadded genetic locus confers one or more traits such as, for example,herbicide tolerance, insect resistance, disease resistance, and modifiedcarbohydrate metabolism. In further embodiments, the trait may beconferred by a naturally occurring gene introduced into the genome of aline by backcrossing, a natural or induced mutation, or a transgeneintroduced through genetic transformation techniques into the plant or aprogenitor of any previous generation thereof. When introduced throughtransformation, a genetic locus may comprise one or more genesintegrated at a single chromosomal location.

The invention also concerns the seed of tomato hybrid PS01533588 and/ortomato lines FDR 15-2090 and FDR 15-2078. The tomato seed of theinvention may be provided as an essentially homogeneous population oftomato seed of tomato hybrid PS01533588 and/or tomato lines FDR 15-2090and FDR 15-2078. Essentially homogeneous populations of seed aregenerally free from substantial numbers of other seed. Therefore, seedof hybrid PS01533588 and/or tomato lines FDR 15-2090 and FDR 15-2078 maybe defined as forming at least about 97% of the total seed, including atleast about 98%, 99% or more of the seed. The seed population may beseparately grown to provide an essentially homogeneous population oftomato plants designated PS01533588 and/or tomato lines FDR 15-2090 andFDR 15-2078.

In yet another aspect of the invention, a tissue culture of regenerablecells of a tomato plant of hybrid PS01533588 and/or tomato lines FDR15-2090 and FDR 15-2078 is provided. The tissue culture will preferablybe capable of regenerating tomato plants capable of expressing all ofthe physiological and morphological characteristics of the startingplant, and of regenerating plants having substantially the same genotypeas the starting plant. Examples of some of the physiological andmorphological characteristics of the hybrid PS01533588 and/or tomatolines FDR 15-2090 and FDR 15-2078 include those traits set forth in thetables herein. The regenerable cells in such tissue cultures may bederived, for example, from embryos, meristems, cotyledons, pollen,leaves, anthers, roots, root tips, pistils, flowers, seed and stalks.Still further, the present invention provides tomato plants regeneratedfrom a tissue culture of the invention, the plants having all thephysiological and morphological characteristics of hybrid PS01533588and/or tomato lines FDR 15-2090 and FDR 15-2078.

In still yet another aspect of the invention, processes are provided forproducing tomato seeds, plants and fruit, which processes generallycomprise crossing a first parent tomato plant with a second parenttomato plant, wherein at least one of the first or second parent tomatoplants is a plant of tomato line FDR 15-2090 or tomato line FDR 15-2078.These processes may be further exemplified as processes for preparinghybrid tomato seed or plants, wherein a first tomato plant is crossedwith a second tomato plant of a different, distinct genotype to providea hybrid that has, as one of its parents, a plant of tomato line FDR15-2090 or tomato line FDR 15-2078. In these processes, crossing willresult in the production of seed. The seed production occurs regardlessof whether the seed is collected or not.

In one embodiment of the invention, the first step in “crossing”comprises planting seeds of a first and second parent tomato plant,often in proximity so that pollination will occur for example, mediatedby insect vectors. Alternatively, pollen can be transferred manually.Where the plant is self-pollinated, pollination may occur without theneed for direct human intervention other than plant cultivation.

A second step may comprise cultivating or growing the seeds of first andsecond parent tomato plants into plants that bear flowers. A third stepmay comprise preventing self-pollination of the plants, such as byemasculating the flowers (i.e., killing or removing the pollen).

A fourth step for a hybrid cross may comprise cross-pollination betweenthe first and second parent tomato plants. Yet another step comprisesharvesting the seeds from at least one of the parent tomato plants. Theharvested seed can be grown to produce a tomato plant or hybrid tomatoplant.

The present invention also provides the tomato seeds and plants producedby a process that comprises crossing a first parent tomato plant with asecond parent tomato plant, wherein at least one of the first or secondparent tomato plants is a plant of tomato hybrid PS01533588 and/ortomato lines FDR 15-2090 and FDR 15-2078. In one embodiment of theinvention, tomato seed and plants produced by the process are firstgeneration (F₁) hybrid tomato seed and plants produced by crossing aplant in accordance with the invention with another, distinct plant. Thepresent invention further contemplates plant parts of such an F₁ hybridtomato plant, and methods of use thereof. Therefore, certain exemplaryembodiments of the invention provide an F₁ hybrid tomato plant and seedthereof.

In still yet another aspect, the present invention provides a method ofproducing a plant derived from hybrid PS01533588 and/or tomato lines FDR15-2090 and FDR 15-2078, the method comprising the steps of: (a)preparing a progeny plant derived from hybrid PS01533588 and/or tomatolines FDR 15-2090 and FDR 15-2078, wherein said preparing comprisescrossing a plant of the hybrid PS01533588 and/or tomato lines FDR15-2090 and FDR 15-2078 with a second plant; and (b) crossing theprogeny plant with itself or a second plant to produce a seed of aprogeny plant of a subsequent generation. In further embodiments, themethod may additionally comprise: (c) growing a progeny plant of asubsequent generation from said seed of a progeny plant of a subsequentgeneration and crossing the progeny plant of a subsequent generationwith itself or a second plant; and repeating the steps for an additional3-10 generations to produce a plant derived from hybrid PS01533588and/or tomato lines FDR 15-2090 and FDR 15-2078. The plant derived fromhybrid PS01533588 and/or tomato lines FDR 15-2090 and FDR 15-2078 may bean inbred line, and the aforementioned repeated crossing steps may bedefined as comprising sufficient inbreeding to produce the inbred line.In the method, it may be desirable to select particular plants resultingfrom step (c) for continued crossing according to steps (b) and (c). Byselecting plants having one or more desirable traits, a plant derivedfrom hybrid PS01533588 and/or tomato lines FDR 15-2090 and FDR 15-2078is obtained which possesses some of the desirable traits of theline/hybrid as well as potentially other selected traits.

In certain embodiments, the present invention provides a method ofproducing food or feed comprising: (a) obtaining a plant of tomatohybrid PS01533588 and/or tomato lines FDR 15-2090 and FDR 15-2078,wherein the plant has been cultivated to maturity, and (b) collecting atleast one tomato from the plant.

In still yet another aspect of the invention, the genetic complement oftomato hybrid PS01533588 and/or tomato lines FDR 15-2090 and FDR 15-2078is provided. The phrase “genetic complement” is used to refer to theaggregate of nucleotide sequences, the expression of which sequencesdefines the phenotype of, in the present case, a tomato plant, or a cellor tissue of that plant. A genetic complement thus represents thegenetic makeup of a cell, tissue or plant, and a hybrid geneticcomplement represents the genetic make up of a hybrid cell, tissue orplant. The invention thus provides tomato plant cells that have agenetic complement in accordance with the tomato plant cells disclosedherein, and seeds and plants containing such cells.

Plant genetic complements may be assessed by genetic marker profiles,and by the expression of phenotypic traits that are characteristic ofthe expression of the genetic complement, e.g., isozyme typing profiles.It is understood that hybrid PS01533588 and/or tomato lines FDR 15-2090and FDR 15-2078 could be identified by any of the many well knowntechniques such as, for example, Simple Sequence Length Polymorphisms(SSLPs) (Williams et al., 1990), Randomly Amplified Polymorphic DNAs(RAPDs), DNA Amplification Fingerprinting (DAF), Sequence CharacterizedAmplified Regions (SCARs), Arbitrary Primed Polymerase Chain Reaction(AP-PCR), Amplified Fragment Length Polymorphisms (AFLPs) (EP 534 858,specifically incorporated herein by reference in its entirety), andSingle Nucleotide Polymorphisms (SNPs) (Wang et al., 1998).

In still yet another aspect, the present invention provides hybridgenetic complements, as represented by tomato plant cells, tissues,plants, and seeds, formed by the combination of a haploid geneticcomplement of a tomato plant of the invention with a haploid geneticcomplement of a second tomato plant, preferably, another, distincttomato plant. In another aspect, the present invention provides a tomatoplant regenerated from a tissue culture that comprises a hybrid geneticcomplement of this invention.

In still yet another aspect, the invention provides a plant of an hybridtomato that exhibits a combination of traits comprising a determinategrowth habit; resistance to Fusarium wilt race 3, tomato spotted wiltvirus, and tomato yellow leaf curl virus; and a good yield ofextra-large, medium-red colored, medium-firm fruit. In certainembodiments, the combination of traits may be defined as controlled bygenetic means for the expression of the combination of traits found intomato hybrid PS01533588.

In still yet another aspect, the invention provides a method ofdetermining the genotype of a plant of tomato hybrid PS01533588 and/ortomato lines FDR 15-2090 and FDR 15-2078 comprising detecting in thegenome of the plant at least a first polymorphism. The method may, incertain embodiments, comprise detecting a plurality of polymorphisms inthe genome of the plant. The method may further comprise storing theresults of the step of detecting the plurality of polymorphisms on acomputer readable medium. The invention further provides a computerreadable medium produced by such a method.

Any embodiment discussed herein with respect to one aspect of theinvention applies to other aspects of the invention as well, unlessspecifically noted.

The term “about” is used to indicate that a value includes the standarddeviation of the mean for the device or method being employed todetermine the value. The use of the term “or” in the claims is used tomean “and/or” unless explicitly indicated to refer to alternatives onlyor the alternatives are mutually exclusive. When used in conjunctionwith the word “comprising” or other open language in the claims, thewords “a” and “an” denote “one or more,” unless specifically notedotherwise. The terms “comprise,” “have” and “include” are open-endedlinking verbs. Any forms or tenses of one or more of these verbs, suchas “comprises,” “comprising,” “has,” “having,” “includes” and“including,” are also open-ended. For example, any method that“comprises,” “has” or “includes” one or more steps is not limited topossessing only those one or more steps and also covers other unlistedsteps. Similarly, any plant that “comprises,” “has” or “includes” one ormore traits is not limited to possessing only those one or more traitsand covers other unlisted traits.

Other objects, features and advantages of the present invention willbecome apparent from the following detailed description. It should beunderstood, however, that the detailed description and any specificexamples provided, while indicating specific embodiments of theinvention, are given by way of illustration only, since various changesand modifications within the spirit and scope of the invention willbecome apparent to those skilled in the art from this detaileddescription.

BRIEF DESCRIPTION OF THE DRAWINGS

FIG. 1 shows growth of hybrid PS01533588 tomato plants in a plot with aheavy and uniform natural infection with fusarium wilt race 3.

FIG. 2. Shows growth of Pik ripe 461 tomato plants in a plot with aheavy and uniform natural infection with fusarium wilt race 3.

DETAILED DESCRIPTION OF THE INVENTION

The invention provides methods and compositions relating to plants,seeds and derivatives of tomato hybrid PS01533588, tomato line FDR15-2090 and tomato line FDR 15-2078. The hybrid PS01533588 is producedby the cross of parent lines FDR 15-2090 and FDR 15-2078. The parentlines show uniformity and stability within the limits of environmentalinfluence. By crossing the parent lines, uniform seed of hybridPS01533588 can be obtained.

The development of tomato hybrid PS01533588 and its parent lines can besummarized as follows.

A. ORIGIN AND BREEDING HISTORY OF TOMATO HYBRID PS01533588

The parents of hybrid PS01533588 are FDR 15-2090 and FDR 15-2078. Thedevelopment of FDR 15-2090 can be summarized as follows:

-   Year 1 The fresh market hybrid tomato ‘Floralina’ was made by    crossing fresh market tomato inbred FL 7547 [resistant to race 3    fusarium wilt [Fusarium oxysporum f. sp. Lycopersici] which was    licensed from the University of Florida by Asgrow with tomato inbred    NC 84173.-   Year 2 Hybrid tomato Floralina fruits were bulked to make F2 seed.-   Year 3 Five trays totaling 300 individual F2 plants were screened    for resistance to race 3 fusarium wilt by live inoculation of    spores. Inoculation was on 5 week-old seedlings. Two weeks post    inoculation, plants were indexed for survival. A total of 203 plants    were rated as resistant or intermediate resistant. The best 48    plants with an R rating which showed no sign of fusarium infection    were transplanted into a survivor greenhouse. Of these, the top 5    horticultural selections were made as single plant selections. The    above-described protocol was repeated with half the population [150    total plants], resulting in the selection of the top 3 individual F3    survivors out of the family with the best horticultural type which    included healthy vigorous plant, good fruit set, large to mostly    extra-large fruit size, and good color and firmness.-   Year 4 A molecular marker available for the identification and    selection of traits is the marker for the I-3 gene for Fusarium wilt    race 3 resistance. The F4 of the three F3 selections were planted    and tissue from two week-old seedlings was harvested for DNA    extraction. Two of the F4 plants were identified as fixed for the    I-3 gene and the third was identified as heterozygous. Following    transplantation, one of the fixed F4 plants was self-pollinated    while another plant was crossed with inbred NC 84173 in order to    increase fruit size. This outcross was basically a BC1F1.    Subsequently, the F4 Floralina X NC 84173 F1 hybrid was    self-pollinated to generate F2 seeds.-   Year 5 300 BC1F2 seedlings were selected via marker-assisted    selection [MAS] with the I-3 molecular marker. Only heterozygous    individuals were selected for transplant. Fifty heterozygous [HET]    selections were transplanted into the greenhouse. The top 7    horticultural selections for extra-large fruit size and best    firmness were selected. The above process was subsequently repeated    with the BC1F3 generation.-   Year 6 The above-described MAS strategy was repeated for the BC1F4.    HET selections were compared with individuals fixed [R] for the I-3    gene in the BC1F5 generation since the BC1F4 population appeared    fairly uniform. Finding no significant differences in fruit size or    firmness between the best HET and R selections, the top 3 R BC1F5    selections were harvested.-   Year 7 In addition to being put into a test crossing block, the top    three BC1F6 individuals were evaluated to select the best candidate    for advancement into foundation seed [FS]. The top BC1F7 elite    inbred was transferred to foundation seed and given the final    designation FDR 15-2090. Harvested foundation seed source was FTO    8682-04. Fusarium wilt race 3 resistance was confirmed in two    independent live pathological tests as well as a repeat check in the    molecular marker lab.-   Year 8 FDR 15-2090 is an elite Fusarium race 3-resistant parent    useful for hybrid seed production. The of fruit firmness is good.    Fruit size is very close to NC 84173 which is an important trait in    all fresh market tomato markets in the Americas. Plant health and    vigor, fruit set, size, firmness, and color as well as all other key    agronomic features have proven stable in subsequent generations.

The development of FDR 15-2078 can be summarized as follows:

-   Year 1 Hybrid 200396 made with a cross of line 411 [Tomato spotted    wilt resistant] X line 186J [jointless stem attachment]. Line 411 is    a derivative from South Africa and line 186J is a derivative from a    Florida breeding program [Petoseed Co.]-   Year 1 F2 bulk harvest from hybrid 200396 made from plot 191.-   Year 2 Fixed jointless single plant selection source 97 Cul 783-1    made in F3 from segregating F2 plot. Both parents are resistant to    Verticillium wilt race 1 and Fusarium wilt races 1 and 2, so will be    resistant in all selections made in the F2. The selection was also    uniform green at the mature green stage [line 186J has a green    shoulder]-   Year 2 Tomato spotted wilt virus [TSWV] resistance fixed in single    plant selection in F4 generation of inbred 411/186J. Also tested    resistant to Alternaria stem canker and Grey leaf spot in Pathology    lab.-   Year 3 F5 bulk of inbred 411/186J, source 98 CUL 148 BS. Line is    already completely uniform for all traits.-   Year 3 Final bulk in F6 generation of inbred 411/186J, source 98 FH    163 BS.-   Year 3 Original hybrid PSR 150938 made between FDR-15-2031    [411/186J] and FDR-16-2045 (U.S. Pat. No. 6,414,226).-   Year 4 First FS increase of FDR-15-2031 made with 100 bulked plants,    source FTO 8244-99. Observed to be uniform for large plant, large    deep oblate firm red fruits and jointless stem attachment. Source    was 98 FH 163 BS.-   Year 4 F2 bulk made from hybrid PSR 150938, stake number 251, source    98 SE 3265 [from Salama, Guatemala].-   Year 5 Single plant selection source 00 Cul 250-1 made in the F2    generation for large, healthy plant and good set of firm Large to    extra large red fruits. Verticillium wilt race 1, Fusarium wilt    races 1 and 2, and Alternaria stem canker are determined to be fixed    following testing in the pathology lab.-   Year 6 Single plant selection made in F3 generation from 00 Cul    250-1 single plant selection. Plant is a very healthy, tall    Determinate with large to extra large firm red fruits with good    color. ToMV, TSWV, and TYLCV are determined by molecular marker    testing to all still be segregating [heterozygous].-   Year 6 Fifteen single plant selections made of ‘938RR’ for vigorous    plant, and uniform large firm fruits. Following molecular marker    analysis for tomato spotted wilt {Sw-5 gene] and tomato yellow leaf    curl [Ty-1 gene], selection 01 GHH 200-15 is determined to be fixed    for both genes.-   Year 8 First FS increase of F5 ‘938RR’ made as line code    FDR-15-2078, source FTO-8612-03. Minor rouging out of smaller    fruits.-   Year 9 Second FS increase of F6 bulk of FDR-15-2078 from source    FTO-8612-03. New source FTO 8694-04. Plant and fruit and observed to    be complexly uniform.

The parent lines are uniform and stable, as is a hybrid therefrom. Asmall percentage of variants can occur within commercially acceptablelimits for almost any characteristic during the course of repeatedmultiplication. However no variants are expected.

B. PHYSIOLOGICAL AND MORPHOLOGICAL CHARACTERISTICS OF TOMATO HYBRIDPS01533588 AND TOMATO LINE FDR 15-2090

In accordance with one aspect of the present invention, there isprovided a plant having the physiological and morphologicalcharacteristics of tomato hybrid PS01533588 and the parent linesthereof. A description of the physiological and morphologicalcharacteristics of such plants is presented in Tables 1-2.

TABLE 1 Physiological and Morphological Characteristics of HybridPS01533588 CHARACTERISTIC PS01533588 Cultivation conditions Greenhouseor field? Field Direct-seeded or transplanted? Transplanted Staked orunstaked? Staked Seedling Anthocyanin in hypocotyl of 2-15 cm seedlingPresent Habit of 3-4 week old seedling Normal Mature Plant (at maximumvegetative development) Height 120 cm Growth Determinate Form NormalSize of canopy (compared to others of similar Medium type) HabitSprawling (decumbent) Stem Branching Intermediate (Westover) Branchingat cotyledonary or first leafy node Absent Number of nodes between firstinflorescence 1-4 Number of nodes between early (1^(st)-2^(nd), 2^(nd)-4-7 3^(rd)) inflorescences Number of nodes between later developing 4-7inflorescences Pubescence on younger stems Sparsely hairy (scatteredlong hairs) Leaf (mature leaf beneath the 3^(rd) inflorescence) TypeTomato Morphology Bi-pinnate; normal tomato Margins of major leafletsShallowly toothed or scalloped Marginal rolling or wiltiness SlightOnset of leaflet rolling Late season Surface of major leaflets SmoothPubescence Normal Inflorescence (3^(rd) inflorescence) Type SimpleAverage number of flowers in inflorescence 7   Leafy or “running”inflorescence Absent Flower Calyx Normal (lobes awl shaped) Calyx-lobesApprox. equaling corolla Corolla color Yellow Style pubescence SparseAnthers All fused into tube Fasciation (1^(st) flower of 2^(nd) or3^(rd) inflorescence) Absent Fruit (3^(rd) fruit of 2^(nd) or 3^(rd)cluster) Typical fruit shape Circular Shape of transverse section RoundShape of stem end Flat Shape of blossom end Flat Shape of pistil scarDot Abscission layer Present (pedicellate) Point of detachment of fruitat harvest At pedicel joint Length of dedicel 10 mm Length of maturefruit (stem axis) 70 mm Diameter of fruit at widest point 80 mm Weightof mature fruit 285 grams Number of locules Five or more Fruit surfaceSmooth Fruit base color (mature-green stage) Light green (Lanai, VF145-F5) Fruit pattern (mature-green stage) Uniform green Shoulder colorif different from base Not different from base Fruit color, full-ripeRed Flesh color, full-ripe Red/Crimson Flesh color Uniform Locular gelcolor of table-ripe fruit Red Ripening (blossom-to-stem axis) UniformRipening (central-to-peripheral radial axis) Uniform Stem scar sizeLarge Core Present Epidermis color Yellow Epidermis Normal Epidermistexture Average Thickness of pericarp 10 mm Anthocyanin in hypocotyl of2-15 cm seedling Present Habit of 3-4 week old seedling NormalResistance to Fruit Disorder Cracking, concentric Resistant Disease andPest Reaction Viral Diseases Cracking, concentric Resistant Cucumbermosaic Susceptible Tobacco mosaic, Race 0 Susceptible Tobacco mosaic,Race 1 Susceptible Tobacco mosaic, Race 2 Susceptible Tobacco mosaic,Race2² Susceptible Tomato spotted wilt Resistant Tomato yellow (TYLC)Resistant Bacterial Diseases Bacterial canker (CorynebacteriumSusceptible michiganense) Bacterial speck (Pseudomonas tomato)Susceptible Bacterial spot (Xanthomonas vesicatorium) SusceptibleBacterial wilt (Pseudomonas solanacearum) Susceptible Fungal DiseasesEarly blight defoliation (Alternaria solani) Susceptible Fusarium wilt,Race 1 (F. oxysporum f. Resistant lycopersici) Fusarium wilt, Race 2Resistant Fusarium wilt, Race 3 Resistant Gray leaf spot (Stemphyliumspp.) Resistant Late blight, Race 0 (Phytophthora infestans) SusceptibleLate blight, Race 1 Susceptible Verticillium wilt, Race 1 (V.albo-atrum) Resistant Chemistry and Composition of Full-Ripe Fruits Ph4.9 Titratable acidity, as % citric 6.8 Total solids (dry matter, seedsand skin removed) 5.6 (% by weight) Soluble solids as % Brix 5.1Phenology Seeding to 50% flow (1 open on 50% of plants) 58 days Seed toonce over harvest (if applicable) 118    Fruiting season Medium(Westover) Relative maturity in areas tested Medium Adaptation CultureField Principle use(s) Fresh market Machine harvest Not adapted Regionsto which adaptation has been Mid Atlantic; Southeast; Florida;demonstrated Sacramento and Upper San Joaquin Valley of California*These are typical values. Values may vary due to environment. Othervalues that are substantially equivalent are also within the scope ofthe invention.

TABLE 2 Physiological and Morphological Characteristics of Line FDR15-2090 CHARACTERISTIC FDR 15-2090 Cultivation conditions Greenhouse orfield? Field Direct-seeded or transplanted? Transplanted Staked orunstaked? Unstaked Seedling Anthocyanin in hypocotyl of 2-15 cm seedlingPresent Habit of 3-4 week old seedling Normal Mature Plant (at maximumvegetative development) Height 104 cm (staked) Growth Determinate FormNormal Size of canopy (compared to others of similar Medium type) HabitSprawling (decumbent) Stem Branching Intermediate (Westover) Branchingat cotyledonary or first leafy node Absent Number of nodes between firstinflorescence 1-4 Number of nodes between early (1^(st)-2^(nd), 2^(nd)-4-7 3^(rd)) inflorescences Number of nodes between later developing 4-7inflorescences Pubescence on younger stems Sparsely hairy (scatteredlong hairs) Leaf (mature leaf beneath the 3^(rd) inflorescence) TypeTomato Morphology Bi-pinnate; normal tomato Margins of major leafletsShallowly toothed or scalloped Marginal rolling or wiltiness ModerateOnset of leaflet rolling Mid-season Surface of major leaflets SmoothPubescence Normal Inflorescence (3^(rd) inflorescence) Type SimpleAverage number of flowers in inflorescence 6   Leafy or “running”inflorescence Absent Flower Calyx Normal (lobes awl shaped) Calyx-lobesApprox. equaling corolla Corolla color Yellow Style pubescence SparseAnthers All fused into tube Fasciation (1^(st) flower of 2^(nd) or3^(rd) inflorescence) Absent Fruit (3^(rd) fruit of 2^(nd) or 3^(rd)cluster) Typical fruit shape Circular Shape of transverse section RoundShape of stem end Flat Shape of blossom end Nippled Shape of pistil scarDot Abscission layer Present (pedicellate) Point of detachment of fruitat harvest At pedicel joint Length of dedicel 12 mm Length of maturefruit (stem axis) 81 mm Diameter of fruit at widest point 89 mm Weightof mature fruit 280 grams Number of locules Five or more Fruit surfaceSmooth Fruit base color (mature-green stage) Light green (Lanai, VF145-F5) Fruit pattern (mature-green stage) Uniform green Shoulder colorNot different from base Fruit color, full-ripe Red Flesh color,full-ripe Red/Crimson Flesh color Uniform Locular gel color oftable-ripe fruit Red Ripening (blossom-to-stem axis) Uniform Ripening(central to peripheral radial axis) Uniform Stem scar size Large CorePresent Epidermis color Yellow Epidermis Normal Epidermis textureAverage Thickness of pericarp 11 mm Anthocyanin in hypocotyl of 2-15 cmseedling Absent Habit of 3-4 week old seedling Normal Resistance toFruit Disorder Cracking, concentric Resistant Disease and Pest ReactionViral Diseases Cucumber mosaic Susceptible Tobacco mosaic, Race 0Susceptible Tobacco mosaic, Race 1 Susceptible Tobacco mosaic, Race 2Susceptible Cracking, concentric Resistant Tobacco mosaic, Race2²Susceptible Tomato spotted wilt Susceptible Tomato yellows SusceptibleBacterial Diseases Bacterial canker (Corynebacterium miciganense)Susceptible Bacterial speck (Pseudomonas tomato) Susceptible Bacterialspot (Xanthomonas vesicatorium) Susceptible Bacterial wilt (Pseudomonassolanacearum) Susceptible Fungal Diseases Early blight defoliation(Alternaria solani) Susceptible Fusarium wilt, Race 1 (F. oxysporum f.Resistant lycopersici) Fusarium wilt, Race 2 Resistant Fusarium wilt,Race 3 Resistant Gray leaf spot (Stemphylium spp.) Resistant Lateblight, Race 0 (Phytophthora infestans) Susceptible Late blight, Race 1Susceptible Verticillium wilt, Race 1 (V. albo-atrum) Resistant Insectsand Pests Southern root knot nematode (Meloidogyne Susceptibleincognita) Chemistry and Composition of Full-Ripe Fruits pH 4.31Titratable acidity, as % citric 6.50 Total solids (dry matter, seeds andskin removed) 5.87 (% by weight) Soluble solids as % Brix 5.06 PhenologySeeding to 50% flow (1 open on 50% of plants) 56 days Seed to once overharvest (if applicable) 119 days Fruiting season Medium (Westover)Relative maturity in areas tested Medium Adaptation Culture FieldPrinciple use(s) Fresh market Machine harvest Not adapted Regions towhich adaptation has been Mid Atlantic; Southeast; Florida; demonstratedSacramento and Upper San Joaquin Valley of California *These are typicalvalues. Values may vary due to environment. Other values that aresubstantially equivalent are also within the scope of the invention.

C. BREEDING TOMATO PLANTS

One aspect of the current invention concerns methods for producing seedof tomato hybrid PS01533588 involving crossing tomato lines FDR 15-2090and FDR 15-2078. Alternatively, in other embodiments of the invention,hybrid PS01533588, line FDR 15-2090, or line FDR 15-2078 may be crossedwith itself or with any second plant. Such methods can be used forpropagation of hybrid PS01533588 and/or the tomato lines FDR 15-2090 andFDR 15-2078, or can be used to produce plants that are derived fromhybrid PS01533588 and/or the tomato lines FDR 15-2090 and FDR 15-2078.Plants derived from hybrid PS01533588 and/or the tomato lines FDR15-2090 and FDR 15-2078 may be used, in certain embodiments, for thedevelopment of new tomato varieties.

The development of new varieties using one or more starting varieties iswell known in the art. In accordance with the invention, novel varietiesmay be created by crossing hybrid PS01533588 followed by multiplegenerations of breeding according to such well known methods. Newvarieties may be created by crossing with any second plant. In selectingsuch a second plant to cross for the purpose of developing novel lines,it may be desired to choose those plants which either themselves exhibitone or more selected desirable characteristics or which exhibit thedesired characteristic(s) when in hybrid combination. Once initialcrosses have been made, inbreeding and selection take place to producenew varieties. For development of a uniform line, often five or moregenerations of selfing and selection are involved.

Uniform lines of new varieties may also be developed by way ofdouble-haploids. This technique allows the creation of true breedinglines without the need for multiple generations of selfing andselection. In this manner true breeding lines can be produced in aslittle as one generation. Haploid embryos may be produced frommicrospores, pollen, anther cultures, or ovary cultures. The haploidembryos may then be doubled autonomously, or by chemical treatments(e.g. colchicine treatment). Alternatively, haploid embryos may be growninto haploid plants and treated to induce chromosome doubling. In eithercase, fertile homozygous plants are obtained. In accordance with theinvention, any of such techniques may be used in connection with a plantof the invention and progeny thereof to achieve a homozygous line.

Backcrossing can also be used to improve an inbred plant. Backcrossingtransfers a specific desirable trait from one inbred or non-inbredsource to an inbred that lacks that trait. This can be accomplished, forexample, by first crossing a superior inbred (A) (recurrent parent) to adonor inbred (non-recurrent parent), which carries the appropriate locusor loci for the trait in question. The progeny of this cross are thenmated back to the superior recurrent parent (A) followed by selection inthe resultant progeny for the desired trait to be transferred from thenon-recurrent parent. After five or more backcross generations withselection for the desired trait, the progeny have the characteristicbeing transferred, but are like the superior parent for most or almostall other loci. The last backcross generation would be selfed to givepure breeding progeny for the trait being transferred.

The plants of the present invention are particularly well suited for thedevelopment of new lines based on the elite nature of the geneticbackground of the plants. In selecting a second plant to cross withPS01533588 and/or tomato lines FDR 15-2090 and FDR 15-2078 for thepurpose of developing novel tomato lines, it will typically be preferredto choose those plants which either themselves exhibit one or moreselected desirable characteristics or which exhibit the desiredcharacteristic(s) when in hybrid combination. Examples of desirabletraits may include, in specific embodiments, high seed yield, high seedgermination, seedling vigor, high fruit yield, disease tolerance orresistance, and adaptability for soil and climate conditions.Consumer-driven traits, such as a fruit shape, color, texture, and tasteare other examples of traits that may be incorporated into new lines oftomato plants developed by this invention.

D. PERFORMANCE CHARACTERISTICS

As described above, hybrid PS01533588 exhibits desirable agronomictraits. The performance characteristics of this hybrid were the subjectof an objective analysis of the performance traits relative to othervarieties. The results of the analysis are presented below, and in FIGS.1 and 2.

TABLE 3 Incidence of (“% Infection”) of Geminivirus* for PS01533588 andComparison Varieties Variety % Infection** PS01533588 0 R458 90 R449 95PS01543815 0 Plants were grown as two replicates of ten plants per plot.*“Geminivirus” included but was not limited to: tomato yellow leaf curlvirus, tomato severe leaf curl virus, and pepper hasteco virus. **“%Infection” was calculated as the number of infected plants/20 *100;natural infection was at or very close to 100% during the trial.

TABLE 4 Mean Fruit Size and Weight of Tomatoes with Resistance toFusarium Wilt Race 3 For PS01533588 and Comparison Varieties VarietyFruit Size* Fruit Weight (grams) Sungard  62.5 a**  208 a** FDR 15-209087.0 b 305 b PS01533588 84.5 b 291 b Plants were grown as fourreplicates of ten plants per plot. Mean fruit size and weight werecalculated from 20 randomly-selected full-sized mature red fruitsharvested per plot (total n = 80). *Mean fruit size measured in cm atthe widest point of the fruit cross-sectionally. **Means not sharing thesame letter differ significantly (p = 0.01) according to Duncan'smultiple range test.

TABLE 5 Results of Genotypic and Pathological Tests for Fusarium WiltRace 3 Resistance For PS01533588 and Comparison Varieties Variety I-3Marker Result* Pathology Screen Result* Floralina H 100 F5 FDR 15-2090 R100 F6 FDR 15-2090 R 96.6 F7 FDR 15-2090 R 100 PS01533588 H 100 Pik ripe461 S 0 Flats of 30 plants each were sprayed with live fungal spores ofFusarium wilt race 3 when the seedlings were five weeks old. *I-3 markercorresponds to Fusarium wilt race 3 resistance. I-3 marker is scored asS = susceptible, H = heterozygous, R = resistant. **Plants were ratedfor % resistant based on healthy versus infected tissue four weeks afterspraying.

E. FURTHER EMBODIMENTS OF THE INVENTION

In certain aspects of the invention, plants described herein areprovided modified to include at least a first desired heritable trait.Such plants may, in one embodiment, be developed by a plant breedingtechnique called backcrossing, wherein essentially all of themorphological and physiological characteristics of a variety arerecovered in addition to a genetic locus transferred into the plant viathe backcrossing technique. The term single locus converted plant asused herein refers to those tomato plants which are developed by a plantbreeding technique called backcrossing, wherein essentially all of themorphological and physiological characteristics of a variety arerecovered in addition to the single locus transferred into the varietyvia the backcrossing technique. By essentially all of the morphologicaland physiological characteristics, it is meant that the characteristicsof a plant are recovered that are otherwise present when compared in thesame environment, other than an occasional variant trait that mightarise during backcrossing or direct introduction of a transgene. Oneembodiment of the current invention therefore provides a plant asdescribed herein that comprises a single locus conversion and/ortransgene, wherein the plant comprises essentially all of themorphological and physiological characteristics of tomato hybridPS01533588, tomato line FDR 15-2090, or tomato line FDR 15-2078. It isunderstood in the art that “single locus conversion” encompasses locithat are both transgenic and non-transgenic in their origin.

Backcrossing methods can be used with the present invention to improveor introduce a characteristic into the present variety. The parentaltomato plant which contributes the locus for the desired characteristicis termed the nonrecurrent or donor parent. This terminology refers tothe fact that the nonrecurrent parent is used one time in the backcrossprotocol and therefore does not recur. The parental tomato plant towhich the locus or loci from the nonrecurrent parent are transferred isknown as the recurrent parent as it is used for several rounds in thebackcrossing protocol.

In a typical backcross protocol, the original variety of interest(recurrent parent) is crossed to a second variety (nonrecurrent parent)that carries the single locus of interest to be transferred. Theresulting progeny from this cross are then crossed again to therecurrent parent and the process is repeated until a tomato plant isobtained wherein essentially all of the morphological and physiologicalcharacteristics of the recurrent parent are recovered in the convertedplant, in addition to the single transferred locus from the nonrecurrentparent.

The selection of a suitable recurrent parent is an important step for asuccessful backcrossing procedure. The goal of a backcross protocol isto alter or substitute a single trait or characteristic in the originalvariety. To accomplish this, a single locus of the recurrent variety ismodified or substituted with the desired locus from the nonrecurrentparent, while retaining essentially all of the rest of the desiredgenetic, and therefore the desired physiological and morphologicalconstitution of the original variety. The choice of the particularnonrecurrent parent will depend on the purpose of the backcross; one ofthe major purposes is to add some commercially desirable trait to theplant. The exact backcrossing protocol will depend on the characteristicor trait being altered and the genetic distance between the recurrentand nonrecurrent parents. Although backcrossing methods are simplifiedwhen the characteristic being transferred is a dominant allele, arecessive allele, or an additive allele (between recessive anddominant), may also be transferred. In this instance it may be necessaryto introduce a test of the progeny to determine if the desiredcharacteristic has been successfully transferred.

In one embodiment, progeny tomato plants of a backcross in which a plantdescribed herein is the recurrent parent comprise (i) the desired traitfrom the non-recurrent parent and (ii) all of the physiological andmorphological characteristics of tomato the recurrent parent asdetermined at the 5% significance level when grown in the sameenvironmental conditions.

New varieties can also be developed from more than two parents. Thetechnique, known as modified backcrossing, uses different recurrentparents during the backcrossing. Modified backcrossing may be used toreplace the original recurrent parent with a variety having certain moredesirable characteristics or multiple parents may be used to obtaindifferent desirable characteristics from each.

Many single locus traits have been identified that are not regularlyselected for in the development of a new inbred but that can be improvedby backcrossing techniques. Single locus traits may or may not betransgenic; examples of these traits include, but are not limited to,herbicide resistance, resistance to bacterial, fungal, or viral disease,insect resistance, modified fatty acid or carbohydrate metabolism, andaltered nutritional quality. These comprise genes generally inheritedthrough the nucleus.

Direct selection may be applied where the single locus acts as adominant trait. For this selection process, the progeny of the initialcross are assayed for viral resistance and/or the presence of thecorresponding gene prior to the backcrossing. Selection eliminates anyplants that do not have the desired gene and resistance trait, and onlythose plants that have the trait are used in the subsequent backcross.This process is then repeated for all additional backcross generations.

Selection of tomato plants for breeding is not necessarily dependent onthe phenotype of a plant and instead can be based on geneticinvestigations. For example, one can utilize a suitable genetic markerwhich is closely genetically linked to a trait of interest. One of thesemarkers can be used to identify the presence or absence of a trait inthe offspring of a particular cross, and can be used in selection ofprogeny for continued breeding. This technique is commonly referred toas marker assisted selection. Any other type of genetic marker or otherassay which is able to identify the relative presence or absence of atrait of interest in a plant can also be useful for breeding purposes.Procedures for marker assisted selection are well known in the art. Suchmethods will be of particular utility in the case of recessive traitsand variable phenotypes, or where conventional assays may be moreexpensive, time consuming or otherwise disadvantageous. Types of geneticmarkers which could be used in accordance with the invention include,but are not necessarily limited to, Simple Sequence Length Polymorphisms(SSLPs) (Williams et al., 1990), Randomly Amplified Polymorphic DNAs(RAPDs), DNA Amplification Fingerprinting (DAF), Sequence CharacterizedAmplified Regions (SCARs), Arbitrary Primed Polymerase Chain Reaction(AP-PCR), Amplified Fragment Length Polymorphisms (AFLPs) (EP 534 858,specifically incorporated herein by reference in its entirety), andSingle Nucleotide Polymorphisms (SNPs) (Wang et al., 1998).

F. PLANTS DERIVED BY GENETIC ENGINEERING

Many useful traits that can be introduced by backcrossing, as well asdirectly into a plant, are those which are introduced by genetictransformation techniques. Genetic transformation may therefore be usedto insert a selected transgene into a plant of the invention or may,alternatively, be used for the preparation of transgenes which can beintroduced by backcrossing. Methods for the transformation of plantsthat are well known to those of skill in the art and applicable to manycrop species include, but are not limited to, electroporation,microprojectile bombardment, Agrobacterium-mediated transformation anddirect DNA uptake by protoplasts.

To effect transformation by electroporation, one may employ eitherfriable tissues, such as a suspension culture of cells or embryogeniccallus or alternatively one may transform immature embryos or otherorganized tissue directly. In this technique, one would partiallydegrade the cell walls of the chosen cells by exposing them topectin-degrading enzymes (pectolyases) or mechanically wound tissues ina controlled manner.

An efficient method for delivering transforming DNA segments to plantcells is microprojectile bombardment. In this method, particles arecoated with nucleic acids and delivered into cells by a propellingforce. Exemplary particles include those comprised of tungsten,platinum, and preferably, gold. For the bombardment, cells in suspensionare concentrated on filters or solid culture medium. Alternatively,immature embryos or other target cells may be arranged on solid culturemedium. The cells to be bombarded are positioned at an appropriatedistance below the macroprojectile stopping plate.

An illustrative embodiment of a method for delivering DNA into plantcells by acceleration is the Biolistics Particle Delivery System, whichcan be used to propel particles coated with DNA or cells through ascreen, such as a stainless steel or Nytex screen, onto a surfacecovered with target cells. The screen disperses the particles so thatthey are not delivered to the recipient cells in large aggregates.Microprojectile bombardment techniques are widely applicable, and may beused to transform virtually any plant species.

Agrobacterium-mediated transfer is another widely applicable system forintroducing gene loci into plant cells. An advantage of the technique isthat DNA can be introduced into whole plant tissues, thereby bypassingthe need for regeneration of an intact plant from a protoplast. ModernAgrobacterium transformation vectors are capable of replication in E.coli as well as Agrobacterium, allowing for convenient manipulations(Klee et al., 1985). Moreover, recent technological advances in vectorsfor Agrobacterium-mediated gene transfer have improved the arrangementof genes and restriction sites in the vectors to facilitate theconstruction of vectors capable of expressing various polypeptide codinggenes. The vectors described have convenient multi-linker regionsflanked by a promoter and a polyadenylation site for direct expressionof inserted polypeptide coding genes. Additionally, Agrobacteriumcontaining both armed and disarmed Ti genes can be used fortransformation.

In those plant strains where Agrobacterium-mediated transformation isefficient, it is the method of choice because of the facile and definednature of the gene locus transfer. The use of Agrobacterium-mediatedplant integrating vectors to introduce DNA into plant cells is wellknown in the art (Fraley et al., 1985; U.S. Pat. No. 5,563,055).

Transformation of plant protoplasts also can be achieved using methodsbased on calcium phosphate precipitation, polyethylene glycol treatment,electroporation, and combinations of these treatments (see, e.g.,Potrykus et al., 1985; Omirulleh et al., 1993; Fromm et al., 1986;Uchimiya et al., 1986; Marcotte et al., 1988). Transformation of plantsand expression of foreign genetic elements is exemplified in Choi et al.(1994), and Ellul et al. (2003).

A number of promoters have utility for plant gene expression for anygene of interest including but not limited to selectable markers,scoreable markers, genes for pest tolerance, disease resistance,nutritional enhancements and any other gene of agronomic interest.Examples of constitutive promoters useful for plant gene expressioninclude, but are not limited to, the cauliflower mosaic virus (CaMV)P-35S promoter, which confers constitutive, high-level expression inmost plant tissues (see, e.g., Odel et al., 1985), including in monocots(see, e.g., Dekeyser et al., 1990; Terada and Shimamoto, 1990); atandemly duplicated version of the CaMV 35S promoter, the enhanced 35Spromoter (P-e35S); the nopaline synthase promoter (An et al., 1988); theoctopine synthase promoter (Fromm et al., 1989); and the Figwort mosaicvirus (P-FMV) promoter as described in U.S. Pat. No. 5,378,619 and anenhanced version of the FMV promoter (P-eFMV) where the promotersequence of P-FMV is duplicated in tandem; the Cauliflower mosaic virus19S promoter; a Sugarcane bacilliform virus promoter; a Commelina yellowmottle virus promoter; and other plant DNA virus promoters known toexpress in plant cells.

A variety of plant gene promoters that are regulated in response toenvironmental, hormonal, chemical, and/or developmental signals can alsobe used for expression of an operably linked gene in plant cells,including promoters regulated by (1) heat (Callis et al., 1988), (2)light (e.g., pea rbcS-3A promoter, Kuhlemeier et al., 1989; maize rbcSpromoter, Schaffner and Sheen, 1991; or chlorophyll a/b-binding proteinpromoter, Simpson et al., 1985), (3) hormones, such as abscisic acid(Marcotte et al., 1989), (4) wounding (e.g., wunl, Siebertz et al.,1989); or (5) chemicals such as methyl jasmonate, salicylic acid, orSafener. It may also be advantageous to employ organ-specific promoters(e.g., Roshal et al., 1987; Schernthaner et al., 1988; Bustos et al.,1989).

Exemplary nucleic acids which may be introduced to plants of thisinvention include, for example, DNA sequences or genes from anotherspecies, or even genes or sequences which originate with or are presentin the same species, but are incorporated into recipient cells bygenetic engineering methods rather than classical reproduction orbreeding techniques. However, the term “exogenous” is also intended torefer to genes that are not normally present in the cell beingtransformed, or perhaps simply not present in the form, structure, etc.,as found in the transforming DNA segment or gene, or genes which arenormally present and that one desires to express in a manner thatdiffers from the natural expression pattern, e.g., to over-express.Thus, the term “exogenous” gene or DNA is intended to refer to any geneor DNA segment that is introduced into a recipient cell, regardless ofwhether a similar gene may already be present in such a cell. The typeof DNA included in the exogenous DNA can include DNA which is alreadypresent in the plant cell, DNA from another plant, DNA from a differentorganism, or a DNA generated externally, such as a DNA sequencecontaining an antisense message of a gene, or a DNA sequence encoding asynthetic or modified version of a gene.

Many hundreds if not thousands of different genes are known and couldpotentially be introduced into a tomato plant according to theinvention. Non-limiting examples of particular genes and correspondingphenotypes one may choose to introduce into a tomato plant include oneor more genes for insect tolerance, such as a Bacillus thuringiensis(B.t.) gene, pest tolerance such as genes for fungal disease control,herbicide tolerance such as genes conferring glyphosate tolerance, andgenes for quality improvements such as yield, nutritional enhancements,environmental or stress tolerances, or any desirable changes in plantphysiology, growth, development, morphology or plant product(s). Forexample, structural genes would include any gene that confers insecttolerance including but not limited to a Bacillus insect control proteingene as described in WO 99/31248, herein incorporated by reference inits entirety, U.S. Pat. No. 5,689,052, herein incorporated by referencein its entirety, U.S. Pat. Nos. 5,500,365 and 5,880,275, hereinincorporated by reference in their entirety. In another embodiment, thestructural gene can confer tolerance to the herbicide glyphosate asconferred by genes including, but not limited to Agrobacterium strainCP4 glyphosate resistant EPSPS gene (aroA:CP4) as described in U.S. Pat.No. 5,633,435, herein incorporated by reference in its entirety, orglyphosate oxidoreductase gene (GOX) as described in U.S. Pat. No.5,463,175, herein incorporated by reference in its entirety.

Alternatively, the DNA coding sequences can affect these phenotypes byencoding a non-translatable RNA molecule that causes the targetedinhibition of expression of an endogenous gene, for example viaantisense- or cosuppression-mediated mechanisms (see, for example, Birdet al., 1991). The RNA could also be a catalytic RNA molecule (i.e., aribozyme) engineered to cleave a desired endogenous mRNA product (seefor example, Gibson and Shillito, 1997). Thus, any gene which produces aprotein or mRNA which expresses a phenotype or morphology change ofinterest is useful for the practice of the present invention.

G. DEFINITIONS

In the description and tables herein, a number of terms are used. Inorder to provide a clear and consistent understanding of thespecification and claims, the following definitions are provided:

Allele: Any of one or more alternative forms of a gene locus, all ofwhich alleles relate to one trait or characteristic. In a diploid cellor organism, the two alleles of a given gene occupy corresponding locion a pair of homologous chromosomes.

Backcrossing: A process in which a breeder repeatedly crosses hybridprogeny, for example a first generation hybrid (F₁), back to one of theparents of the hybrid progeny. Backcrossing can be used to introduce oneor more single locus conversions from one genetic background intoanother.

Crossing: The mating of two parent plants.

Cross-pollination: Fertilization by the union of two gametes fromdifferent plants.

Diploid: A cell or organism having two sets of chromosomes.

Emasculate: The removal of plant male sex organs or the inactivation ofthe organs with a cytoplasmic or nuclear genetic factor or a chemicalagent conferring male sterility.

Enzymes: Molecules which can act as catalysts in biological reactions.

F₁ Hybrid: The first generation progeny of the cross of two nonisogenicplants.

Genotype: The genetic constitution of a cell or organism.

Haploid: A cell or organism having one set of the two sets ofchromosomes in a diploid.

Linkage: A phenomenon wherein alleles on the same chromosome tend tosegregate together more often than expected by chance if theirtransmission was independent.

Marker: A readily detectable phenotype, preferably inherited incodominant fashion (both alleles at a locus in a diploid heterozygoteare readily detectable), with no environmental variance component, i.e.,heritability of 1.

Phenotype: The detectable characteristics of a cell or organism, whichcharacteristics are the manifestation of gene expression.

Quantitative Trait Loci (QTL): Quantitative trait loci (QTL) refer togenetic loci that control to some degree numerically representabletraits that are usually continuously distributed.

Resistance: As used herein, the terms “resistance” and “tolerance” areused interchangeably to describe plants that show no symptoms to aspecified biotic pest, pathogen, abiotic influence or environmentalcondition. These terms are also used to describe plants showing somesymptoms but that are still able to produce marketable product with anacceptable yield. Some plants that are referred to as resistant ortolerant are only so in the sense that they may still produce a crop,even though the plants are stunted and the yield is reduced.

Regeneration: The development of a plant from tissue culture.

Self-pollination: The transfer of pollen from the anther to the stigmaof the same plant.

Single Locus Converted (Conversion) Plant: Plants which are developed bya plant breeding technique called backcrossing, wherein essentially allof the morphological and physiological characteristics of a tomatovariety are recovered in addition to the characteristics of the singlelocus transferred into the variety via the backcrossing technique and/orby genetic transformation.

Substantially Equivalent: A characteristic that, when compared, does notshow a statistically significant difference (e.g., p=0.05) from themean.

Tissue Culture: A composition comprising isolated cells of the same or adifferent type or a collection of such cells organized into parts of aplant.

Transgene: A genetic locus comprising a sequence which has beenintroduced into the genome of a tomato plant by transformation.

H. DEPOSIT INFORMATION

A deposit of tomato hybrid PS01533588 and inbred parent line FDR15-2090, disclosed above and recited in the claims, has been made withthe American Type Culture Collection (ATCC), 10801 University Blvd.,Manassas, Va. 20110-2209. The date of the deposits was Jan. 26, 2010.The accession numbers for those deposited seeds of tomato hybridPS01533588 and inbred parent line FDR 15-2090 are ATCC Accession No.PTA-10608 and ATCC Accession No. PTA-10602, respectively. Upon issuanceof a patent, all restrictions upon the deposits will be removed, and thedeposits are intended to meet all of the requirements of 37 C.F.R.§1.801-1.809. The deposits will be maintained in the depository for aperiod of 30 years, or 5 years after the last request, or for theeffective life of the patent, whichever is longer, and will be replacedif necessary during that period.

Although the foregoing invention has been described in some detail byway of illustration and example for purposes of clarity andunderstanding, it will be obvious that certain changes and modificationsmay be practiced within the scope of the invention, as limited only bythe scope of the appended claims.

All references cited herein are hereby expressly incorporated herein byreference.

REFERENCES

The following references, to the extent that they provide exemplaryprocedural or other details supplementary to those set forth herein, arespecifically incorporated herein by reference:

-   U.S. Pat. No. 5,378,619-   U.S. Pat. No. 5,463,175-   U.S. Pat. No. 5,500,365-   U.S. Pat. No. 5,563,055-   U.S. Pat. No. 5,633,435-   U.S. Pat. No. 5,689,052-   U.S. Pat. No. 5,880,275-   An et al., Plant Physiol., 88:547, 1988.-   Bird et al., Biotech. Gen. Engin. Rev., 9:207, 1991.-   Bustos et al., Plant Cell, 1:839, 1989.-   Callis et al., Plant Physiol., 88:965, 1988.-   Choi et al., Plant Cell Rep., 13: 344-348, 1994.-   Dekeyser et al., Plant Cell, 2:591, 1990.-   Ellul et al., Theor. Appl. Genet., 107:462-469, 2003.-   EP 534 858-   Fraley et al., Bio/Technology, 3:629-635, 1985.-   Fromm et al., Nature, 312:791-793, 1986.-   Fromm et al., Plant Cell, 1:977, 1989.-   Gibson and Shillito, Mol. Biotech., 7:125, 1997-   Klee et al., Bio-Technology, 3(7):637-642, 1985.-   Kuhlemeier et al., Plant Cell, 1:471, 1989.-   Marcotte et al., Nature, 335:454, 1988.-   Marcotte et al., Plant Cell, 1:969, 1989.-   Odel et al., Nature, 313:810, 1985.-   Omirulleh et al., Plant Mol. Biol., 21(3):415-428, 1993.-   Potrykus et al., Mol. Gen. Genet., 199:183-188, 1985.-   Roshal et al., EMBO J., 6:1155, 1987.-   Schaffner and Sheen, Plant Cell, 3:997, 1991.-   Schernthaner et al., EMBO J., 7:1249, 1988.-   Siebertz et al., Plant Cell, 1:961, 1989.-   Simpson et al., EMBO J., 4:2723, 1985.-   Terada and Shimamoto, Mol. Gen. Genet., 220:389, 1990.-   Uchimiya et al., Mol. Gen. Genet., 204:204, 1986.-   Wang et al., Science, 280:1077-1082, 1998.-   Williams et al., Nucleic Acids Res., 1 8:6531-6535, 1990.-   WO 99/31248.

1. A tomato plant comprising at least a first set of the chromosomes oftomato line FDR 15-2090, a sample of seed of said line having beendeposited under ATCC Accession No. PTA-10602.
 2. A seed comprising atleast a first set of the chromosomes of tomato line FDR 15-2090, asample of seed of said line having been deposited under ATCC AccessionNo. PTA-10602.
 3. The plant of claim 1, which is hybrid.
 4. A plant partof the plant of claim
 1. 5. The plant part of claim 4, further definedas a leaf, an ovule, pollen, a fruit, or a cell.
 6. The plant part ofclaim 5, further defined as a fruit.
 7. A tissue culture of regenerablecells of the plant of claim
 1. 8. The tissue culture according to claim7, comprising cells or protoplasts from a plant part selected from thegroup consisting of embryos, meristems, cotyledons, pollen, leaves,anthers, roots, root tips, pistil, flower, seed and stalks.
 9. A tomatoplant regenerated from the tissue culture of claim
 8. 10. A method ofvegetatively propagating the plant of claim 1 comprising the steps of:(a) obtaining tissue capable of being propagated from the plantaccording to claim 1; (b) cultivating said tissue to obtain proliferatedshoots; and (c) rooting said proliferated shoots to obtain rootedplantlets.
 11. The method of claim 10, further comprising growing plantsfrom said rooted plantlets.
 12. A method of introducing a desired traitinto a tomato line comprising: (a) crossing a plant of line FDR 15-2090,a sample of seed of said line having been deposited under ATCC AccessionNumber PTA-10602, with a second tomato plant that comprises a desiredtrait to produce F1 progeny; (b) selecting an F1 progeny that comprisesthe desired trait; (c) crossing the selected F1 progeny with a plant ofline FDR 15-2090 to produce backcross progeny; and (d) repeating steps(b) and (c) three or more times to produce selected fourth or higherbackcross progeny that comprises the desired trait.
 13. A tomato plantproduced by the method of claim
 12. 14. A method of producing a plantcomprising a transgene, the method comprising introducing a transgeneinto a plant of tomato line FDR 15-2090, a sample of seed of said linehaving been deposited under ATCC Accession No. PTA-10602.
 15. A plant oftomato line FDR 15-2090 further comprising a transgene, a sample of seedof said line having been deposited under ATCC Accession No. PTA-10602.16. A seed that produces the plant of claim
 15. 17. A plant of tomatoline FDR 15-2090 further comprising a single locus conversion, a sampleof seed of said line having been deposited under ATCC Accession No.PTA-10602, wherein said plant contains essentially all of themorphological and physiological characteristics of tomato line FDR15-2090.
 18. A seed that produced the plant of claim
 17. 19. A methodfor producing a seed of a plant derived from line FDR 15-2090 comprisingthe steps of: (a) crossing a tomato plant of line FDR 15-2090 with asecond tomato plant; a sample of seed of said line having been depositedunder ATCC Accession Number PTA-10602; and (b) allowing seed of a lineFDR 15-2090-derived tomato plant to form.
 20. The method of claim 19,further comprising the steps of: (c) crossing a plant grown from saidFDR 15-2090-derived tomato seed with itself or a different tomato plantto yield additional FDR 15-2090-derived tomato seed; (d) growing saidadditional FDR 15-2090-derived tomato seed of step (c) to yieldadditional FDR 15-2090-derived tomato plants; and (e) repeating thecrossing and growing steps of (c) and (d) to generate at least a firstfurther FDR 15-2090-derived tomato plant.
 21. The method of claim 19,wherein the second tomato plant is of an inbred tomato line.
 22. Themethod of claim 20, further comprising: (f) crossing the further FDR15-2090-derived tomato plant with another tomato plant to produce seedof a hybrid progeny plant.
 23. A method of producing a tomato fruitcomprising: (a) obtaining the plant according to claim 1, wherein theplant has been cultivated to maturity; and (b) collecting a tomato fromthe plant.
 24. A method of producing seed comprising crossing the plantof claim 1 with itself or a second plant.